He used different enzymes to cleave the hemoglobin from sickle celled and normal individuals. What is the most likely diagnosis for Michael. He recognized that these results indicated it was likely that there was a difference in one or more molecules found either in the blood or within the red blood cells.
All of the discoveries mentioned in Section 1 were made by the individuals they are attributed to, as were the observations made by Dr. The following gel was obtained: What class of amino acid is valine. At low oxygen pressure, deoxy-HbS polymerises and gets organised in long polymer fibres that deform, stiffen, and weaken the red blood cell not shown.
Be sure to give the basis for your answer. He also tested this using red blood cell ghosts. Today he was starting a new graduate student in the lab.
This case is a work of fiction that refers to real events and people. The others are the controls. It appears to have a number of interesting structural properties. That one nucleotide difference however causes a change in the amino acid sequence where a Valine is incorporated in HbS while a Glutamate is incorporated in HbA.
After analyzing that fragment, he determined that the only difference between normal and sickle-cell hemoglobin is the amount of glutamic acid and valine residues. Any errors should be attributed to the author and not the original investigators.
Although the writing was difficult to make out, it appeared that he was to test a mM solution of sucrose, a mM solution of NaCl, a mM solution of NaCl, and a saline solution. Determine the tonicity of each of the solutions tested.
Oxygenated hemoglobin does not present a hydrophobic surface to other hemoglobin molecules, so polymerization is much less likely in the oxygenated state. As a faculty member of Harvard Medical School, he had always received a fair amount of inquiries, but after he had published his data indicating that pernicious anemia was due to a vitamin B12 deficiency, the amount of mail he received was sometimes overwhelming.
The type of electrophoresis we performed separated the proteins based on their overall electrical charge. Castle was out the door. Understanding Sickle Cell Disease. Why would a Glu Val mutation cause so many problems in the functioning of the protein.
Describe what should happen to the red blood cells after they were placed into each of the solutions. She does report feeling fatigued and has been having burning with urination along with urinating frequently. I do hope that you still have an interest in sickle cell I feel that I would make a positive addition to your lab.
After repeating this process four times, the average time for sickling to occur was: Hemoglobin from sickle-celled individuals contains more valine.
Originally published September 14, ; last revised February 15, Do you know what a saline solution is composed of. This process represents the basic mechanisms leading to haemolytic anaemia and to vaso-occlusive events in the microcirculation. From these results, what level s of protein structure of the hemoglobin is altered in the sickled-cell condition.
HbS has a different overall net charge than HbA, which causes it to run differently through the gel. Since sickle cell anemia was named due to the change in the shape of the red blood cells, the molecule involved was most likely within the red blood cells.
Dr. Castle placed the letter in a conspicuous place on his desk so that he would remember to send a reply to the ambitious student. NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCE. Sickle Cell Anemia: A Fictional Reconstruction* by Debra Stamper Department of Biology King's College.
NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCE “Sickle Cell Anemia” by Debra Stamper Page 5 Eagerly Dr. Castle reached for his lab notebook and quickly looked to see which samples came from the individuals with sickle cell anemia.
“Jim, come and look at these results Linus Pauling just sent to me,” he called to his research assistant. Search Sickle Cell Anemia A Fictional Reconstruction Answer PDF window or a Find toolbar. While fundamental function consult with by the 2 alternatives is pretty.
Start studying Sickle Cell Anemia Case Study. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Page 1 NATIONAL CENTER FOR CASE STUDY TEACHING IN SCIENCE Case Teaching Notes for “Sickle Cell Anemia” by Debra Stamper INTRODUCTION / BACKGROUND Th is case was designed to be implemented in the fi rst.Sickle cell anemia a fictional reconstruction case study answers